Multiplexed IHC: Has anyone ever observed that the antigen retrieval conditions for one antibody might be affecting the staining of another antibody ?

Hi all, I am trying to combine 4 different markers for my multiplexed IHC. Their Antigen Retrieval (AR) conditions are as follows: Marker A: pH9 microwave, 30 mins Marker B: pH9 microwave, 15...

27 November 2017 6,127 3 View