Teca Galvão

4 Questions 5 Answers 0 Followers

Questions related from Teca Galvão

Using FAM labelled oligos in fluorescence anisotropy and EMSA for looking at protein-DNA interaction?

Hi everyone, I have doubts about oligos used in fluorescence anisotropy for looking at protein-DNA interaction. Do both oligos need to have FAM at the 5' end? (I guess the positive side of...

10 December 2021 3,773 1 View

Does the lambda red Datsenko system leave stop codons after removal of the resistance cassette?

Hi, When using the lambda red system, after removing the antibiotic cassette, scar sequences are left behind. Do these sequences vary from clone to clone? Do they contain stop codons? In the three...

05 July 2021 9,630 4 View

What filter type would you use for a protein in buffer that contains 1% DMSO?

If you need to filter 500 ul of a protein that´s in a buffer with 1% DMSO, how would you filter this before putting in an analytical equipment, like for SPR? Would you use regenerated cellulose...

03 October 2019 9,638 5 View

Are there new fast methods for single base change in the E coli chromosome?

Hi everyone, I need to make a single base change (no scars or markers can be left) in the E. coli chromosome and would like to avoid using the traditional method (clone, insert in genome,...

27 August 2019 8,358 6 View