ELISPOT: Why do I see IgE spots in bone marrow of both control mice and T-cell dep. immunized mice?

I performed an ELISPOT for IgE using a biotinylated IgE-antibody and Streptavidin-ALP for detection of spots. I used bone marrow cells of two wild-type mice: one mouse was immunized with NP-CGG...

12 September 2018 6,287 0 View