Taylor Pini

3 Questions 8 Answers 0 Followers

Questions related from Taylor Pini

ImageJ - Automatically excluding co-localized staining from fluorescence intensity measurement?

I've stained some mouse embryos with a stain for oxidative protein modification (FTSC), and counter stained with DAPI. I'm specifically interested in quantifying extra-nuclear staining, so I'm...

06 June 2019 5,595 4 View

Plate choice for immuno-PCR?

I'm trying to develop an immuno-PCR (ELISA, followed by PCR detection of oligo), but am struggling to find a compatible plate type. I've tried Nunc Top Yield strips from Thermo, which are supposed...

10 October 2018 8,273 6 View

Do smaller than expected column fractions mean an issue with the pump or the column?

I'm currently running a HiPrep 16/60 sephacryl S-100 column which has some compaction due to running at too high a flow rate. Since this occurred, I have reversed the flow direction to help ease...

03 March 2016 1,701 3 View