Taylor Pini

3 Questions 10 Answers 0 Followers

Questions related from Taylor Pini

Recommended methods for derivatization of protein carbonyl modifications for mass spec?

I'm preparing sperm lysates for mass spec, and I'm specifically interested in looking at oxidative modifications to proteins. I'd like to derivatize and enrich these samples prior to mass spec to...

11 July 2018 9,497 0 View

Do smaller than expected column fractions mean an issue with the pump or the column?

I'm currently running a HiPrep 16/60 sephacryl S-100 column which has some compaction due to running at too high a flow rate. Since this occurred, I have reversed the flow direction to help ease...

30 March 2016 4,033 3 View

Are heparin and EDTA vacutainers equally useful as the primary collection tube for neutrophil isolation?

I'm using the protocol linked below, which calls for blood collection into an EDTA laced vacutainer. I'm waiting on arrival of EDTA vacutainers, but I have heparinised ones laying around and I'm...

19 August 2015 7,548 4 View