Tatyana Budylin

4 Questions 8 Answers 0 Followers

Questions related from Tatyana Budylin

Is this DiI staining valid or specific?

I placed DiI crystals into the hilus of P10 mice horizontal sections (vibratome). I think the first image is from the CA3 region, and I can't tell if these are dendrites/axons or background,...

02 February 2016 9,221 18 View

What is the peak of synaptogenesis and neurogenesis in mice?

I have found toxicology research that suggests that it's P10 in mice for synaptogenesis, and other research which suggests that it goes on even into the first or second month. For neurogenesis I...

01 January 2016 2,182 4 View

Does anyone have a good protocol for staining brains with DiI crystals?

Looking for a protocol for DiI staining. Lower concentrations of PFA seem preferable with vibratome sectioning. Is there a preferable way to deliver crystals to the hippocampus or brain in...

01 January 2016 9,270 6 View

Can anyone help me tell which of my doublecortin antibodies is more specific?

I am using one antibody from cell signaling, which seems to stain projections from the granular zone and stains in between the BRDU positive cells, and i'm using a different doublecortin antibody...

05 May 2015 7,895 5 View