Tatiana Takahasi Komoto

3 Questions 3 Answers 0 Followers

Questions related from Tatiana Takahasi Komoto

Has anyone ever have a problem with Validation data from mi-seq?

I performed a Mi-seq experiments using cancer cell lines, and as a result a large number of different microRNA showed different modulation levels. Afterthat, based on these data I chose some miRNA...

10 October 2018 800 3 View

Why after induction THP-1 monocyte to macrophage using the PMA as inductor, the RNA gel showed 3 bands of rRNA?

I’m working with induction of THP-1 monocyte to macrophage using 12 ng/mL of PMA as inductor, but when I realize the RNA extraction appeared 3 bands on gel, Why is it happens?

05 May 2018 2,070 2 View

How I can to do the MTT assay in a plant cell?

I want to do the viability assay using MTT salt in a plant cell, but I don't know how I can count these cells, can I use the same method to the human cell?

11 November 2016 8,143 4 View