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Questions related from Tassanee Lerksuthirat
I have tried to clone the 15kb plasmid (ligation product between 10kb and 5kb - cut gel extract). But after transformation into E.coli DH10beta and do the restriction analysis, we did not get the...
02 September 2016 2,256 4 View
I am curious whether I can short cut the protocol by directly using the antartic phosphatase (NEB) treated plasmid to the ligation without prior purification to remove salt/protein? Does anyone...
01 March 2016 5,750 3 View