Tassanee Lerksuthirat

3 Questions 6 Answers 0 Followers

Questions related from Tassanee Lerksuthirat

Can the mercury-light source lamp be used as the emission near-far red wavelength?

Since the price of Xe-lamp is very expensive for us and we would like to take the image from Alexa flour 647. Have anyone tried to take the immunofluorescence image using the Hg lamp as the light...

13 May 2017 1,773 2 View

How to reduce the recombination in the E.coli clone?

I have tried to clone the 15kb plasmid (ligation product between 10kb and 5kb - cut gel extract). But after transformation into E.coli DH10beta and do the restriction analysis, we did not get the...

02 September 2016 2,291 4 View

Is it necessary to purify the antartic phosphatase (NEB) treated plasmid before further ligation with the insert?

I am curious whether I can short cut the protocol by directly using the antartic phosphatase (NEB) treated plasmid to the ligation without prior purification to remove salt/protein? Does anyone...

01 March 2016 5,782 3 View