Tassanee Lerksuthirat

2 Questions 5 Answers 0 Followers

Questions related from Tassanee Lerksuthirat

How to reduce the recombination in the E.coli clone?

I have tried to clone the 15kb plasmid (ligation product between 10kb and 5kb - cut gel extract). But after transformation into E.coli DH10beta and do the restriction analysis, we did not get the...

02 September 2016 2,258 4 View

Is it necessary to purify the antartic phosphatase (NEB) treated plasmid before further ligation with the insert?

I am curious whether I can short cut the protocol by directly using the antartic phosphatase (NEB) treated plasmid to the ligation without prior purification to remove salt/protein? Does anyone...

01 March 2016 5,752 3 View