Tasnim Ramadan Matarid

4 Questions 4 Answers 0 Followers

Questions related from Tasnim Ramadan Matarid

What is the optimal technique for preparing fatty acid Solution?

I am currently working on establishing a diabetes model by inducing insulin resistance using palmitic acid (PA). To prepare the PA, I dissolved a calculated amount in absolute ethanol to create a...

02 September 2024 5,845 0 View

Why do I have low 260/280 and 260/230 ratios with TRIzol-based RNA extraction?

I am extracting RNA from human adipose tissues using the TRIzol method, following the attached protocol for tissue extraction. The problem is that the 260/280 ratio of my samples is low in most...

11 June 2024 4,658 3 View

Why my HepG2 cell grow very slow?

I am growing my HepG2 cells in low-glucose DMEM (1 g/l glucose) with 10% FBS and 1% anti-anti in a T25 flask. The cells were passaged into a T75 flask after they had grown and clustered, as shown...

27 February 2024 2,368 2 View

Is it normal for HepG2 to have a slow growth rate in low glucose medium?

I have recently begun working with the HepG2 cell line to induce insulin resistance using a high-glucose environment. I acquired the cells from a -80°C storage where they had been since 2019, and...

11 February 2024 1,186 3 View