Tasaduq Hussain Wani

5 Questions 33 Answers 0 Followers

Questions related from Tasaduq Hussain Wani

How much of Gamma H2AX foci is it possible to be found at basal level of any cancer cell line?

Please exclude the case of melanoma which have higher levels of basal gamma-H2AX. And apart from drugs how much can handling of a cell line affect the foci formation in the same?

10 October 2015 4,326 2 View

What could the possible reasons be for variation in protein sizes in loaded lysate in a western blot?

I am providing my western blot image developed by ECL. Lane 1 is the marker and Lane 2 is my recombinant His-Tag Ni-NTA purified (control +ve, 24KDa). Lane 3 and others are the lysates loaded...

03 March 2014 8,504 5 View

How can we exclude TM regions in a protein sequence, when there are many of them in it?

I have to produce antibody for a protein. Upon analysis I found out that there is presence of a signal sequence and nine TM regions. I am confused about, how to exclude all those TM regions when...

11 November 2013 4,332 3 View

Can anybody suggest a way to overcome degradation or loss of protein after dialysis?

Recently I purified one recombinant protein by urea based method (Ni-NTA was used for binding). Initially before dialysis I had a good concentration of protein, but after dialysis I lost my...

08 August 2013 8,294 11 View

Normally OD comes to 0.6 in 1-2 hours, but in my case it takes 5-6 hours for OD to come to 0.5-0.6. Why is it so?

I transformed one of my constructs into BL21 DE3 for recombinant protein production. The gene of interest has been cloned into TOPO vector with proper orientation. Everything is fine with this...

08 August 2013 3,724 27 View