Tarun Tyagi

3 Questions 25 Answers 0 Followers

Questions related from Tarun Tyagi

Can anyone help with a problem in fluorescence detection by a plate reader?

I am trying to optimized a fluorescence based spectrometric assay by 96 well plate method which i was doing using cuvette. But the readings are not being consistent and acceptable. The plate I'm...

24 May 2014 1,630 10 View

How to denature pre-mRNA for RT-PCR ?

mRNA are easy to denature but denaturation of pre-mRNA requires additional steps. I have tried heating at 70C and 80C for 10 minutes prior to cDNA synthesis, but it hasn't worked yet, can...

30 October 2013 8,564 1 View

Is it possible to isolate WBCs or PBMCs from whole blood by Ficoll method without contamination of platelets, and if yes, how ?

I have tried isolating leukocytes from whole blood by Histopaque (sigma), but when I checked it in microscope, I found lots of platelets along with leukocytes, I tried washing, but it din't help much.

21 July 2013 5,301 1 View