Tarana Arman

5 Questions 4 Answers 0 Followers

Questions related from Tarana Arman

Can somebody suggest me an exact role of riboflavin when carrying out a Superoxide Dismutase activity assay?

I want to effect of metals on Superoxide Dismutase activity. Many protocols have suggested the use of riboflavin. However, I am not exactly clear in its role. Alternatively can anyone suggest me a...

17 April 2015 3,663 11 View

Can you please suggest a most reliable protocol for Cellulase zymography?

I am using Bacillus subtilis as a source for cellulase production grown on 0.1% CMC (Carboxy-Methyl-Cellulose) media. And now I want to detect the presence of cellulase using 0.1% congo red stain...

27 March 2015 9,530 6 View

What is the maximum time period for fluorescence of DCF remains?

I am using carboxy-H2DCFDA to detect ROS in Saccharomyces cerevisiae. I will load the dye before incubating my cells with the desired agonist. What is the desired time period till when I can...

09 February 2015 6,375 3 View

Do I need to add PMSF (Serine protease inhibitor) separately even after adding the protease inhibitor cocktail?

I am isolating proteins from yeasts.

03 February 2015 2,480 11 View

I want to do a structural comparison of yeast and human metallothionein using bioinformatics tools. Can anybody suggest a suitable tool?

I want to analyse whether a human metallothionein standard for HPLC can be used for yeast metallothionein as well.

21 January 2015 3,714 1 View