Tarana Arman

7 Questions 5 Answers 0 Followers

Questions related from Tarana Arman

Can somebody suggest me an exact role of riboflavin when carrying out a Superoxide Dismutase activity assay?

I want to effect of metals on Superoxide Dismutase activity. Many protocols have suggested the use of riboflavin. However, I am not exactly clear in its role. Alternatively can anyone suggest me a...

17 April 2015 3,685 11 View

Can you please suggest a most reliable protocol for Cellulase zymography?

I am using Bacillus subtilis as a source for cellulase production grown on 0.1% CMC (Carboxy-Methyl-Cellulose) media. And now I want to detect the presence of cellulase using 0.1% congo red stain...

27 March 2015 9,572 6 View

What is the minimum amount of DHE (Dihydroethidium) that could be used to detect the superoxide ions in saccharomyces cerevisiae?

I have induced a chromium stressed condition for the yeast culture and want to assess the ROS increase in them. However, I am unable to decide upon the upper and lower limits for the amount of DHE...

05 March 2015 6,186 3 View

Can anybody help me in understanding the link between neurodevelopment and gastrointestinal tract development?

In many neurodevelopmental disorders, it has been observed that one of the primary etiology is an underdeveloped gut system. It will be really helpful if somebody can shed light on this.

25 February 2015 8,016 4 View

What is the maximum time period for fluorescence of DCF remains?

I am using carboxy-H2DCFDA to detect ROS in Saccharomyces cerevisiae. I will load the dye before incubating my cells with the desired agonist. What is the desired time period till when I can...

09 February 2015 6,389 3 View

Do I need to add PMSF (Serine protease inhibitor) separately even after adding the protease inhibitor cocktail?

I am isolating proteins from yeasts.

03 February 2015 2,571 11 View

I want to do a structural comparison of yeast and human metallothionein using bioinformatics tools. Can anybody suggest a suitable tool?

I want to analyse whether a human metallothionein standard for HPLC can be used for yeast metallothionein as well.

21 January 2015 3,734 1 View