Taran Khanam

4 Questions 10 Answers 0 Followers

Questions related from Taran Khanam

What does it mean when Kd is several fold lower than Km?

I am studying the DNA binding and kinetic properties of a protein. I found that the Kd value for binding a DNA substrate are much lower than the respective Km value. Is this observation suggestive...

29 January 2015 2,591 8 View

Which software can be used to determine dissociation constant for enzyme-ligand binding using QUADRATIC equation as shown in the pic attached?

I need to calculate the KD for enzyme-ligand using the quadratic equation as shown in the pic. Graph pad prism or origin software do not have these equation by default. However when I am trying to...

20 January 2015 2,834 9 View

Why doesn't 100 percent band shift occur in EMSA even when binding saturation has been achieved?

I am studying DNA binding of a protein using EMSA. The reaction mixture contains 5 nM of DNA substrate while I vary the protein concentration from 2 to 50 nM. I see an increase in band shift with...

17 January 2015 6,629 11 View

Can NaBH4 DNA trapping experiment be effectively done using 5' 6FAM (fluorescently labelled) DNA. Any specific protocol for the same?

I want to check AP lyase activity of my protein using sodium borohydride DNa trapping methods but I cannot order radioactive ATP to label my DNA presently, due to certain limitations..So I was...

20 December 2014 5,901 2 View