Tao Wu

5 Questions 1 Answers 0 Followers

Questions related from Tao Wu

Can Vav-iCre mediate different gene deletion efficiency between fetal and adult endothelial cells?

Published work has shown that Vav-iCre can mediated gene deletion in the endothelial cells, but in our own experiment, we found no significant deletion efficiency in the KO intestinal endothelial...

15 March 2019 744 1 View

Any good tips for intracellular staining?

Hi there, when doing the intracellular cytokine staining for FACS analysis, take IL-17 for example, can the stimulate cells be fixed and permeabilized by Fix/Perm buffer in the presence of...

08 February 2017 2,101 2 View

Does anyone have the experience of the Whole-mount immunostaining for small intestine?

I am trying to detect the Peyer’s Patch Anlagen via the whole-mount immunostaining of the small intestine. But the protocals I found in the papers are briefly illustrated, does anyone have the...

08 February 2017 9,246 1 View

Hi, when I isolate the immune cells from the small intestine, I found that I can't get the cells from the whitened intestine, does anybody know why?

Hi all, when I isolate the immune cells from the lamina propria, after the first media digestion (RPMI 1640, 5mM EDTA, 20mM HEPES, for 20 minutes, 37 C ), I found that some intestine becomes...

07 October 2016 2,031 2 View

Is expression of ST2 on the ILC2 influenced by the collagenase digestion in small intestine lamina propria?

Small intestine lamina propria ILC2 is defined as Lin-CD127+Sca1+ST2+KLRG1+, but what we get is the cells Lin-CD127+Sca1+KLRG1+, while ST2-

26 March 2015 3,805 0 View