Tanuka Sen

3 Questions 3 Answers 0 Followers

Questions related from Tanuka Sen

How to find bacterial promotors from the sequence?

Can someone suggest any reliable bioinformatic tool to search bacterial promotors from the bacterial genome sequence ?

01 January 2019 3,713 2 View

Does anyone know how can I increase the competency of my competent cells ??

Im making competent cells from Shigella with pkD46 plasmid already inside it. Im then electroporating them to get donor DNA go inside them. The efficiency of recombination is pretty low for...

08 August 2018 7,047 4 View

How can I remove the chance of false positives while using Lamda red recombination system for bacterial gene knockouts ?

Im using pkD46 transformed competent cells and transforming with DNA template containing kanamycin resistance gene amplified. I used pkD4 for amplifying the Kanamycin gene. During my...

07 July 2018 7,761 2 View