Why my protein of interest appeared lower molecular weight than expected molecular weight after cleavage of GST tag?

I prepared GST-tagged protein. The molecular weight of my protein (50kda) with GST (26 kDa) tag is 76 kDa. After removal of GST tag the molecular weight should be 50 kDa but in SDS page I got 40...

29 November 2023 6,185 3 View