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Questions related from Tang Xiaoqiang
Now I need to determine the subcellular localization of a given lncRNA with qPCR after cellular fractionation.However,I am not sure whether same amount(e.g.1ug) or same proportion(e.g.1%) of...
06 June 2017 1,018 8 View
The attached is a γH2AX western blotting result.293T cells were treated with etoposide or DMSO(control),then cells were lysed in RIPA buffer with sonication.0.2 nitrocelluose membrane was used for...
04 April 2017 2,658 4 View
I am trying to establish the in vitro ubiquitination to screen the compounds,the reaction system(E1,E2,ATP and reaction buffer) is the same as generally used,and the unreacted(remaining) ATP was...
08 August 2016 6,398 3 View
Does anyone have experimence how long can the sigma etoposide(E1383) power store at RT?And what abou the etoposide solution in DMSO?Do I have to prepare immediatly before use or can I store the...
05 May 2016 653 2 View
As stated,why the gene locus given by the RNA-Seq is different from that of NCBI and ensemble?For mouse fos,the annoted locus for RNA-Seq data is chr12:86814850-86818219,while in NCBI and Ensemble...
02 February 2016 7,011 1 View
As stated,the doubling time for my sf9 cells were increased:the cells were seeded at the density of 106/ml and three days later,it only reached 2*106/ml with higher than 90% viability.The cells...
01 January 2016 968 2 View
I transfected low-passage healthy Sf9 cells with PCR-verified bacmid(Bac-to-Bac system) and saw all the signs of infections during transfection and baculovirus amplication of P1 and P2,but I could...
11 November 2015 4,367 8 View
