Tanaz Onsi̇nejad

2 Questions 3 Answers 0 Followers

Questions related from Tanaz Onsi̇nejad

Hi, Anyone could help me for LMMP and whole mount protocol of rat stomach and intestine tissues?

For our LMMP protocol, first I placed the tissues in phosphate-buffered saline (PBS; 0.1 M, pH = 7.4) containing niphedipine for 15 min, then the tissues were fixated in 4% PFA in at 4 °C...

14 December 2023 2,898 0 View

Anyone could help me regarding rolling and wrinkling problem during frozen sectioning of rat brains via sledge microtome Leica SM2010 ?

Following fixation for 18-24h I store the whole brains in PBS containing Na-azide at 4C. On the sectioning day, I placed the brain on the freezing stage using couple drops of OCT applied to the...

12 September 2023 1,094 5 View