Tami Coursey

3 Questions 5 Answers 0 Followers

Questions related from Tami Coursey

Anyone know of a program for Arabidopsis In silico PCR analysis (whole genome)?

Recently I saw that the Sol Genomics webpage has taken down the option of doing In silico PCR for Arabidopsis. While I have found many programs that allow you to utilize an input sequence, these...

08 August 2016 9,476 4 View

How can I elute my protein from a native antibody without inhibiting protein activity?

I have an antibody against my protein of interest that I would like to use to help with purification. Unfortunately I haven't been able to find a lot of information about how to separate the...

12 December 2014 1,762 6 View

How does one elute an HA-tagged protein from an HA-column if it is binding non-specifically?

I am transiently expressing a HA-His N terminally tagged protein in Nicotiana benthamiana leaves and have been running into problems with purification. I first used a Ni-NTA column and...

10 October 2014 698 7 View