Does anybody have experience with the detection of a FLAG epitope on a Western blot with the Sigma Anti-FLAG antibody M2 after stripping the blot?

I stripped several western blots with 62.5mM Tris-HCl (pH 6.8), containing ß-mercaptoethanol and 2% SDS for 1h at 50°C. After this the FLAG epitope bound to my protein was not detectable anymore...

14 August 2020 8,530 3 View