Takeshi Nakamura

3 Questions 4 Answers 0 Followers

Questions related from Takeshi Nakamura

How can we increase the percentage of moving mitochondria in axons stained with MitoTracker?

We are staining hippocampal neurons with MitoTracker Red and performing time-lapse imaging of mitochondria in axons. But unfortunately, " The percentage of moving mitochondria is considerably less...

18 August 2023 1,744 0 View

How can you fix the cells in Matrigel?

My lab performs three-dimensional culture of neuronal cell lines using 50% Matrigel. Usually, the 50% concentration of Matrigel is used as a condition that promotes efficient neurite outgrowth....

15 January 2018 3,988 2 View

How can a fluorescence recovery of GFP occur after a fluorescence decrease happens by light exposure?

We have observed a fluorescence recovery some minutes after a fluorescnce decrease by a light exposure of GFP and its derivatives some minutes some minutes after a fluorescnce decrease by a light...

31 January 2015 4,729 6 View