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We are staining hippocampal neurons with MitoTracker Red and performing time-lapse imaging of mitochondria in axons. But unfortunately, " The percentage of moving mitochondria is considerably less...
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My lab performs three-dimensional culture of neuronal cell lines using 50% Matrigel. Usually, the 50% concentration of Matrigel is used as a condition that promotes efficient neurite outgrowth....
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