Taghreed Hirz

2 Questions 3 Answers 0 Followers

Questions related from Taghreed Hirz

Can anyone help in finding a protocol for manual dissociation of a tumor tissue for single cell suspensions for RNAseq?

We used an enzymatic dissociation procedure (collagenases) in our previous experiments to dissociate tumor tissue for FACS sorting and then RNAseq but it seems that the enzymatic digestion is...

13 April 2018 2,625 1 View

In-vitro Antigen presentation assay using NSCLC and OT1 CD8 cells. How to perform ova-pulsed target cells and should I activate the CD8 T cells?

I want to perform an in vitro Antigen presentation assay using NSCLC and OT1 CD8 T cells. First I want to know if it is better to pulse the NSCLC cells with OVA257-264 peptide or with the complete...

19 April 2016 9,695 6 View