Tabinda Salman

4 Questions 6 Answers 0 Followers

Questions related from Tabinda Salman

Does any receptor mRNA decrease of less than 5% result in decreased functional receptors' level expressed on the membrane surface?

As we sometimes see a statistically significant difference does not necessarily mean a difference in actual function. My question is does a receptor mRNA (like 5ht1a) decrease of less than 5%...

14 April 2021 1,016 2 View

How to quantify any specific protein expression via any software/application in confocal microscopic images ?

I want to quantify/count neuronal cells, pretty well filled up with the expression signal of a protein. The images have been taken via confocal microscopy at 10x resolution. Which...

26 March 2020 3,608 2 View

What is the criteria for selecting loading control in qPCR?

What is the criteria of selecting GAPDH or Beta-actin as a loading control in real time PCR? What should be considered more in general?

09 March 2015 1,759 8 View

Is the DNase treatment required before the cDNA synthesis?

After RNA extraction, when do we do the gel electrophoresis? Besides the bands of the RNA there is a light band of a genomic DNA as well. Do we need to treat it with DNases so we can further go...

13 February 2015 1,590 10 View