Can anyone advise on fixation and preservation of artery rings for ulterior DAF2 AM fluorescence measurement?

cross sections of aortic rings are incubated with DAF2 AM (10µM, KREBS HEPES pH7.4 for 1 hour). then aortic rings are washed and challenged with various agonists for 1 hour. the resulting DAF 2...

05 May 2015 9,265 4 View