Syeda Fatima

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Questions related from Syeda Fatima

Can anyone help me with phusion site directed mutagenesis ligation process?

Hello, I used this kit for direct mutagenesis and my Ligation step sometimes works and sometimes doesn't. Can someone please tell me what worked for them? Protocol: PCR Product: 10-20ng (I...

28 August 2020 5,077 2 View

How many units of T4 Polynucleotide Kinase can I use in phosphorylation of primers?

I have heard that you could 10 units for a cold reaction and 20 units for a hot labeling reaction. Can I use more than 10 units in a cold reaction? or these numbers should b followed strictly?

06 July 2020 2,485 0 View