12 Questions 15 Answers 0 Followers
Questions related from Syeda Mahreen Hassan
I have used Colab; but every time it got failed .Is the computer specification is necessary for this ?
02 July 2023 4,660 0 View
Do I need to add ampicillin in starter culture if miniprep or do I need to add kanamycin also because my plasmid (pQ30) contains ampicillin resistance gene and M15 pREP4 contains...
15 September 2021 4,447 3 View
I want to use E. faecalis JH22 as competent cells to express my protein using anhydrotetracycline (ATc) to induce protein expression i need some standard protocol if someone has use it before.
27 July 2019 2,090 2 View
Tx6119( M15 containing fragment of SwpA26-226 from TX82 cloned into pQE30),when i run it as control without injecting IPTG i never saw any band on SDS-gel.
02 October 2017 5,574 3 View
TX6119- Fragments were cloned into the expressionvector pQE30 (Qiagen) and transformed into M15 (pREP4) cells(Qiagen).i want to make culture in LB broth to do miniprep i am confuse how much i add...
09 August 2017 1,978 3 View
Positive and negative control
02 June 2017 3,447 3 View
BL21(DE 3)or need to subcluture it
22 May 2017 3,338 6 View
growing the bacteria(LB broth) for protein expression: the optical density(1.2) , is this good for protein expression(before IPTG).
05 April 2017 7,747 12 View
proteomics and molecular biologist
28 February 2017 5,767 5 View
proteomics
20 December 2016 8,000 3 View
please give me formula
27 April 2015 2,130 5 View
i have design the primer for gene synthesis,i have to do gibson assembly i have no idea about PCR,which type of PCR i used
01 January 1970 1,096 3 View