Syeda Mahreen Hassan

11 Questions 14 Answers 0 Followers

Questions related from Syeda Mahreen Hassan

Can anyone tell me how i predict protein interaction through Alpha fold2 for free?

I have used Colab; but every time it got failed .Is the computer specification is necessary for this ?

02 July 2023 4,643 0 View

In starter culture for the miniprep i have to antibiotic which is resistant genes is present on plasmid or do also consider competent cells ?

Do I need to add ampicillin in starter culture if miniprep or do I need to add kanamycin also because my plasmid (pQ30) contains ampicillin resistance gene and M15 pREP4 contains...

15 September 2021 4,437 3 View

I want to do protein expression in E. faecalis JH22 by using anhydrotetracycline (ATc) to induce protein expression .Need protocol for expression?

I want to use E. faecalis JH22 as competent cells to express my protein using anhydrotetracycline (ATc) to induce protein expression i need some standard protocol if someone has use it before.

27 July 2019 2,067 2 View

I want to express my TX6119 strain ;i run it as control without injecting IPTG i saw no band?

Tx6119( M15 containing fragment of SwpA26-226 from TX82 cloned into pQE30),when i run it as control without injecting IPTG i never saw any band on SDS-gel.

02 October 2017 5,560 3 View

How much i add amipicillin and kanamycin into pQE30 (Qiagen) contains cloned fragment and transformed into M15 ( (pREP4) cells to do miniprep?

TX6119- Fragments were cloned into the expressionvector pQE30 (Qiagen) and transformed into M15 (pREP4) cells(Qiagen).i want to make culture in LB broth to do miniprep i am confuse how much i add...

09 August 2017 1,969 3 View

What type of positive and negative control;can be use for Histag antibody in Western blot?

Positive and negative control

02 June 2017 3,437 3 View

Can i use one month old transformation for protein expression?

BL21(DE 3)or need to subcluture it

22 May 2017 3,328 6 View

Growing the bacteria(LB broth) for protein expression: the optical density(1.2) , is this good for protein expression(before IPTG).

growing the bacteria(LB broth) for protein expression: the optical density(1.2) , is this good for protein expression(before IPTG).

05 April 2017 7,736 12 View

Hi,i did transformation of BL21 and top10 cells with plasmid,when send it for sequncing , mutation occurs in one plasmid and other have no insert?

proteomics and molecular biologist

28 February 2017 5,757 5 View

How can I determine the optical density of Lysozyme from chicken white at 280nm on the spectrophotometer ?

proteomics

20 December 2016 7,989 3 View

For Generation of DNA fragments with overlapping ends by PCR,which type of PCR i will use ?

i have design the primer for gene synthesis,i have to do gibson assembly i have no idea about PCR,which type of PCR i used

01 January 1970 1,085 3 View