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I am trying to establish shTET2 3T3-L1 cell line. The efficiency for mCherry (Control) is very good while my experimental sh is not working well. I use 10 microgram of DNA for transfection. Any...
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I am doing proliferation assay, after i fix the cells with 4% paraform aldehyde, i stain the cells with Crystal Violet for 2 hrs, after washing excessive dye i air dry my plates and then use 10%...
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