Syed Azmal Ali

6 Questions 13 Answers 0 Followers

Questions related from Syed Azmal Ali

Not able to run the CODEML in PAMLX to identify lineages with accelerated evolution?

I am trying to run the pamlX for CODEML but somehow not able to get start the run option. After loading all three files that are .ctl, .phy, and .tree, the pamlX program stands still and the RUN...

02 February 2020 4,257 2 View

How to determine the relative quantification of Kinase proteins?

I want to identify the relative quantification of all kinase proteins present in my 6 sample (sample is from rat tissue). Does anyone have any idea how can I perform this experiment? I am looking...

04 April 2017 1,045 4 View

How to determine mitochondrial specific proteins exclusively in total mass spectrometer data?

I have performed the mass spectrometer run along with deep fractionation and identified around 6000 proteins. I want to perform the mitochondrial specific gene enrichment analysis and interested...

02 February 2017 8,491 5 View

How to determine the molecular weight and charge state of protein in top down proteomics using the envelop chromatogram?

I have recently started working on top-down proteomics and while working obtains many chromatograms of envelope shapes. Even the software has the option to deconvolute and identifies the molecular...

02 February 2017 6,791 2 View

How to perform Label check in case of TMT and iTRAQ experiment in Maxquant or other free available search engine?

Dear all, Hello everyone, I am going to start the experiment with 6 plex TMT for my studies. Anyone, please tell me what column should I look for my label check experiment. Initially, to know my...

08 August 2016 9,795 7 View

How to grow MCF12A cell line ?

I am trying to grow MCF 12A cell line using DMEM/F12 medium with all the hormonal composition except Cholera toxin. The cells are not growing well only a few were adhered and remaining died. Is...

07 July 2016 2,673 4 View