Swetha Sridhar

2 Questions 3 Answers 0 Followers

Questions related from Swetha Sridhar

How can I culture primary fibroblasts from a mouse without euthanizing it?

My lab has some mice that we cannot afford to euthanize. We would like to use ear punch biopsies and tail clips for fibroblasts, but I am not sure if there will be enough cells in each to set up a...

11 November 2018 4,472 3 View

How to determine the accurate functional titer of a FLAG tag lentiviral particle?

I have a plasmid of >10 kb that I have made into particles in HEK293T. Before inserting the virus in mice, I would like to determine the functional titer. I have a p24 ELISA kit, but I was...

05 May 2018 9,277 3 View