Swati Yadav

3 Questions 5 Answers 0 Followers

Questions related from Swati Yadav

Why am I getting very high background with my Secondary antibody diluted in "JUST Buffer"?

I used "JUST buffer" (20mM Sodium acetate, 20mM sodium acetate 9% sucrose, 0.01% polysorbate 20, pH 5.2) for doing my ELISA to check for FcgR2A binding of my antibodies. However, I got a very...

07 December 2018 6,758 4 View

Need a suitable ELISA protocol to test biotinylation of IgG?

I biotinylated IgG using the EZ-LINK kit (Thermo Scientific). I want to test the biotinylation using ELISA. Can someone guide me with a suitable protocol for ELISA?

10 October 2018 550 3 View

Good negative control Ab to my indicator Ab (biotinylayed human IgG ) for use in a competitive ELISA?

I am doing a ELISA to test the competitive binding of test antibodies to human FCgR2A. My indicator antibody is biotinylated human IgG (POLYCLONAL) used at 2ug/ml. The competitive antibodies were...

05 October 2018 479 1 View