Swati Gupta

6 Questions 3 Answers 0 Followers

Questions related from Swati Gupta

Have I to consider the oligomeric status of my protein before calculating its molarity?

Hi, I deal with synthetases which are dimeric. SHould I consider their dimeric mol wt before calculating their molarity or should I go with the usual SDS PAGE mol wt which is monomeric in Kilodaltons

12 December 2018 3,340 0 View

Hello, my protein is undergoing aggregation and eluting in void volume during gel filtration. How can I reduce this ?

After affinity through amylose and TEV cleavage, the protein is aggregated in GPC so I am not getting any active protein out of this. How can I minimise aggregation.

04 April 2018 1,095 0 View

Hello < I am trying to standardize IFA with Plasmodium falciparum parasitized RBCs . Can you help in that?

And am using 1:400 dilution for my protein's antibody. With overnight 4 deg binding. Should I make it 1 hour at RT. I increased tween in PBS from 0.01 to 0.05 % . I got some less background. But...

10 October 2017 1,305 0 View

Can you give me a protocol for GST assay?

I am doing it with the sigma GST assay kit. My blank buffer consisting of PBS, GSH and cDNB is also giving an absorbance. So can you just give me a detailed description in case am making a mistake.

08 August 2017 8,948 2 View

Does HIC help to get rid of chaperones?

Am getting a chaperone at around 63 kD . I have my construct in pETM41. Am purifying with amylose resin and getting a chaperone after affinity. Will HIC after first step clean my protein?

07 July 2017 8,883 5 View

How to get rid of chaperone at around 63 kD during recombinant protein expression in C43 competent cells?

I was inducing with 0.8 mM IPTG and kept 16-18 hrs post induction at 25 degree..am trying to reduce the temp and check the expression ; also lowering the salt and increasing imidazole...

07 July 2017 8,887 2 View