I am doing a PCR for a 650 bp DNA for cloning purpose but getting a PCR product of 400 bp even with different conditions. what can be the reason?

1.Non-specific band at 400 bp, even though by gene size is 650 bp 2.No primer dimer 3.PCR Conditions using right now: Denaturation - 98 degrees (45 sec), ext.- 98 degrees (30 sec)...

25 September 2023 6,956 2 View