Swathy S Anand

2 Questions 5 Answers 0 Followers

Questions related from Swathy S Anand

Minimum how much quantity of insert DNA is needed for a success full ligation transformation ?

Hello everyone, I am planning to do a cloning experiment. Can anyone suggest what is the minimum amount of insert DNA and vector DNA required for a 1:7 ligation reaction ? The size of my insert...

18 April 2024 5,807 2 View

Why the gene iserted is not getting amplified after cloning , but it gave postive result for restriction digestion ?

hi everyone, i am trying to figure out why the gene inserted is not amplifying after cloning. I have inserted a mycobacterium-specific gene into pET 32a plasmid and transformed the ligation...

07 April 2024 7,676 8 View