Could anyone suggest any modifications in the protocol for staining of human lung fibroblasts with X-Gal in basal condition for SA-Betagal expression?

I have been trying to stain human lung fibroblasts isolated from lungs of young controls, aged and IPF patients for SA-Betagalactosidase expression. I need them to be in basal condition without...

25 September 2014 6,183 2 View