Swarnendu Chandra

3 Questions 3 Answers 0 Followers

Questions related from Swarnendu Chandra

What is the best way to quantify RNA after isolation?

I am isolating mRNA from leaf tissues for semi-quantitative RT-PCR analysis. I was estimating RNA by spectrophotometrically. But it requires quiet a good amount of RNA. Is there any alternative...

22 June 2014 3,089 16 View

Why formic acid is better than phosphoric acid in HPLC?

People suggest using formic acid for better resolution than phosphoric acid. Is there any specific reason behind it?

27 May 2014 3,844 9 View

What solvent should I use to dissolve kaempferol and quercetin standards for HPLC?

For my experiment I am using quercetin and kaempferol as standards. I am unable to dissolve them in water or methanol, or even in 50% methanol water. Please suggest any alternative solutions for...

22 May 2014 8,170 4 View