Swapnil Srivastava

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Questions related from Swapnil Srivastava

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

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Does age of a bacterial culture have an impact on its DNA isolation?

Generally for bacterial DNA isolation we use a fresh culture of 2-5 days old. But if we use an older culture say 10-15 days old will it have any impact on the DNA content and the DNA isolation...

14 May 2024 2,939 11 View

What concentration of Nystatin should be used in Nutrient Agar medium?

I'm isolating bacterial from soil on Nutrient Agar media but after 4-5 days I see fungal growth start in the plates. I read about use of Nystatin to prevent fungal growth. How much quantity or...

22 April 2024 5,874 5 View

Can 50% glycerol slant be used for storage of Bacterial cultures at -20 C

I do not have -80 C refrigerator for storage of my bacterial cultures. Can they be stored in -20C refrigerators in 50% glycerol slants.

01 January 1970 869 9 View