Swapnil Srivastava

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Questions related from Swapnil Srivastava

Which method of Bacterial Streaking is considered the best and most effective?

While performing Bacterial streaking several methods can be opted like Quadrant, T-method, Continuous, Zigzag etc. Which technique among them is the best and most efficient in obtaining pure cultures.

last month 2,823 7 View

At what OD is the Bacterial Growth in a culture measured, and what if the values are greater than 1?

I have to estimate the growth of my bacterial cultures spectrophotometrically and I read articles of measurement at an OD of 600nm. Also what to do if the values exceed 1. What is the proper...

08 October 2024 8,490 24 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

23 July 2024 9,945 4 View

Does age of a bacterial culture have an impact on its DNA isolation?

Generally for bacterial DNA isolation we use a fresh culture of 2-5 days old. But if we use an older culture say 10-15 days old will it have any impact on the DNA content and the DNA isolation...

14 May 2024 3,163 11 View

What concentration of Nystatin should be used in Nutrient Agar medium?

I'm isolating bacterial from soil on Nutrient Agar media but after 4-5 days I see fungal growth start in the plates. I read about use of Nystatin to prevent fungal growth. How much quantity or...

22 April 2024 6,185 5 View

Can 50% glycerol slant be used for storage of Bacterial cultures at -20 C

I do not have -80 C refrigerator for storage of my bacterial cultures. Can they be stored in -20C refrigerators in 50% glycerol slants.

01 January 1970 912 9 View