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While performing Bacterial streaking several methods can be opted like Quadrant, T-method, Continuous, Zigzag etc. Which technique among them is the best and most efficient in obtaining pure cultures.
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I have to estimate the growth of my bacterial cultures spectrophotometrically and I read articles of measurement at an OD of 600nm. Also what to do if the values exceed 1. What is the proper...
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I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
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Generally for bacterial DNA isolation we use a fresh culture of 2-5 days old. But if we use an older culture say 10-15 days old will it have any impact on the DNA content and the DNA isolation...
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I'm isolating bacterial from soil on Nutrient Agar media but after 4-5 days I see fungal growth start in the plates. I read about use of Nystatin to prevent fungal growth. How much quantity or...
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I do not have -80 C refrigerator for storage of my bacterial cultures. Can they be stored in -20C refrigerators in 50% glycerol slants.
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