Swapna Aravind

2 Questions 14 Answers 0 Followers

Questions related from Swapna Aravind

I am in vitro transcribing a 300 nt RNA using T7 RNA polymerase. I can see the RNA band in an agarose gel but not a 5% polyacrylamide native gel?

If I continue to run the agarose gel longer, the RNA band starts to smear.

01 January 2014 5,081 2 View

Can GST protein be rebound to the resin after elution with GSH ?

I eluted GST from a GST column using 10 mM GSH and removed excess GSH by dialysis. I am aware that GSH remains bound to GST. Does dialysis remove this as well? Will this GST bind the GST-resin again?

10 October 2012 7,596 5 View