Svjatoslavs Kistkins

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Questions related from Svjatoslavs Kistkins

Kobs un case of strong inhibitory effect of the compound - what should we do?

Dear colleagues! Due to the strong irreversible inhibition of the enzyme I have difficulties with the calculation of Kobs. The FRET scan measurement units changes only by 30-60 units during...

10 October 2019 9,968 4 View

The use of medications during osseointegration process. What drugs should we use locally?

Dear colleagues! Can you help to solve a problem? What compounds should we prescribe locally to induce a better osseointegration with implants? Biphosphonates and BMPx (as a GF) are good...

08 August 2019 1,943 9 View

Restriction, ligation of transformation - where is the problem?

Hi everyone! I have a problem with e.coli cultivation. I use pET28a and pBHA plasmids. My gene is in pBHA plasmid pET28a consists of NdeI and XhoI restriction sites. An insert also has the...

07 July 2018 6,970 4 View

The appearance of Negative peaks after positive peaks exiting working with HPLC RI detector. Why does it happen and how can I remove them?

Hi everyone, The problem is that a chromatogram shows negative peaks after positive peak exiting, working with HPLC RI detector. The standard solution and the sample contain carbohydrates. MP: 25%...

02 February 2015 8,902 14 View