Sven Petersen

3 Questions 14 Answers 0 Followers

Questions related from Sven Petersen

GAPDH gene expression is affected by cell stress?

Obviously I have heard about the discussions about the suitability of GAPDH as a control gene for qPCR. But since everyone, so it seems, keeps using it, I didn't bother to change anything. Now,...

02 February 2016 5,499 5 View

Is an MTS assay sensitive enough for radio-sensitisation analysis?

I'm analysing whether a new compound is able to make HCT116 cells more sensitive to X-ray radiation and tested different concentrations in combination with different doses of irradiation. However,...

06 June 2015 8,024 11 View

In flow cytometry compensation using beads/cells why is a very strong single staining of your compensation control of benefit?

I've used beads and cells side by side to do my compensation for a multicolor staining on PBMCs. Here I experienced that the staining (esp. with PE-CD594) on my single stained beads was so strong...

05 May 2014 468 16 View