Does anyone have suggestions about cell density, DIV and surface preparation for hippocampal neuron culturing for micro electrode array?

Actually, I am trying to culture and measure hippocampal neurons from rat's puppies (E18) on MEA neurochip (256-9wellMEA300/30iR-ITO). With 30,000cell/well (~71,000cells/cm²) I could not seen any...

28 November 2014 4,377 2 View