Sven Be

6 Questions 8 Answers 0 Followers

Questions related from Sven Be

Which population should I gate?

Hi, I'm trying to asses the electroporation efficiency of monocyte derived dendritic cells that I differentiated from PBMC monocytes. As you can see from the picture attached, after FACS 4...

06 May 2017 317 6 View

Which statistical test suits best?

Hello everyone, I'm currently trying to find out which statistical test I can use to show differences between two or more populations. I had cells, which I infected with a virus +/- additional...

28 May 2016 6,726 5 View

What is energetically more favorable?

Hey, I've got a question which might be very simple. I was asked which interaction of amino acids is more energetically favorable. My problem begins with the what the definition of "energetically...

01 February 2016 3,014 6 View

Why is the pH increasing during fed-batch fermentation?

Hello everyone, today I started a fed-batch fermentation with E. colis. Shortly after the induction with IPTG I recognized an increase in pH (as the acid pump had to work to keep the pH at 7)....

03 November 2015 4,857 21 View

How do you calculate annealing temperature for bisulfite primer?

Hey guys, I'm trying to do bisulfite sequencing and therefore I designed some primers using MethPrimer. The Tm ist around 59.5 °C for both forward and reverse. Have you experienced a way which...

21 January 2015 5,484 2 View

Does the absence of Oil Red O staining in my MEF adipocytes indicate that there are no adipocytes?

Hey, so within the last week I tried to differentiate wildtype MEF's into adipocytes using adipogenic medium (ADM) containing Insulin, Dexamethason, Indomeathcin, Isobutyxanthin. After 8 days of...

20 January 2015 6,220 5 View