If I perform a digestion of SmaI (NEB) at 37 degrees, more than 1h, can I have the enzyme cutting at non-specific sites?

I have done a digestion with SmaI (NEB) and did not realize the optimal temperature for the reaction is 25 degrees. I left it at 37 degrees for more than 1h, and I got the following...

09 September 2017 953 3 View