Suyeol Im

3 Questions 5 Answers 0 Followers

Questions related from Suyeol Im

How come band different from predicted size is detected?

I analyzed IL-1b in whole cell lysate through western blot and treated LPS as a positive control. Predicted size of IL-1b is 17/31 kD (mature/pro-form). But in the result, band around 40 kD was...

01 September 2017 6,642 3 View

Is there anyone who comment to me about the way for decomposition of NAD(P)?

In many protocols of glucose uptake assay kits or NAD(P)/NAD(P)H assay kits, NAD(P)+ is decomposed by boiling in approx. 80 degrees Celcius. I understood that NAD(P)+ is stable in acidic condition...

20 January 2016 9,457 3 View

What is the reason for immersing the heart in a cold buffer during cardiomyocytes preparation?

In recent papers related with isolation of cardiomyocytes, the heart is immersed in cold KH buffer after excision. In my opinion, it seems like that warm buffer is better than cold one to...

13 April 2014 9,062 12 View