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Hello all, I would like to get some clarity on a fundamental biological process of lenti/retroviruses and stable-cell line generation using 3rd generation system . 1. During the production of...
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Dear All, I have a few questions pertaining to the RNA immunoprecipitation procedure. 1. How do we decide if we need to include a cross-linking step in RIP? 2. If a cross-linking step is...
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Dear all I have a technical question pertaining to molecular biology. Can a RNA binding protein get detected in chromatin immunoprecipitation if the final PCR is designed about the same region in...
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Dear all, I need your valuable suggestion in the following. I am tracking a viral protein in an infected cell. My model cell line is Jurkat T cells which are CD4+ suspension cells. Now, this...
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Dear all, I need your feedback on the right way to analyze flow data for my experiment. I have read articles in a number of blogs and discussion forum about the usage of mean vs median...
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Hello all, I am trying to understand a fundamental concept in molecular biology. Is it possible that the same transcription factor might function both as an activator as well as repressor due to...
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