Sushmita Thakuri

2 Questions 2 Answers 0 Followers

Questions related from Sushmita Thakuri

How do I prevent leaky expression of pAC5.1/V5-His vector in e coli competent cells?

I trying to clone TOP3B in pAC5.1/V5-His vector and express the vector with insert later in Drosophila cells. But when I try to clone TOP3B in pAC5.1/V5-His vector in DH5alpha cells, none of the...

30 May 2025 9,482 2 View

Need troubleshooting suggestions for cloning TOP-3B cDNA in E coli/competent cells ?

Hi, I have been trying to clone topoisomerase-3B from C elegans cDNA in NEB 5-alpha Competent E. coli. I performed hifi assembly of the top-3B cDNA insert and RE digested yeast two-hybrid pGBD...

15 January 2025 8,740 5 View