Sushanta Ratna

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Questions related from Sushanta Ratna

Will the addition of an extra 'T' in the origin of replication of the pGEX-6p1 vector impact protein expression?

While sequencing my pGEX-6p1 vector for protein expression, I discovered an insertion in the origin of replication—five consecutive 'T' residues instead of the original four. As a result, my Ori...

17 February 2025 1,589 2 View

What DNA polymerase is the best for high fidelity?

While I was amplifying a certain gene from chromosomal DNA for my Gibson Cloning, I noticed after sequencing that there is one extra A on the middle of my gene of interest sequence. In the...

03 March 2024 1,109 7 View