Susan Rashid

3 Questions 1 Answers 0 Followers

Questions related from Susan Rashid

What's the best way to remove excess PEG after using it to precipitate phage?

I have been using PEG 8000 (20%) + NaCl to precipitate a clarified phage lysate. After incubating the lysate with the PEG solution, I spin it at 13,000g for 1 hr at 4C. I see the pellets very...

16 January 2023 5,381 3 View

I need to make 100mL of the following recipe: 20mM Tris-HCl, pH 7.5 100mM NaCl 6mM MgCl2. How many grams of NaCl and MgCl2, do I need?

I need to make 100mL of the following recipe: 20mM Tris-HCl, pH 7.5 100mM NaCl 6mM MgCl2. How many grams of NaCl and MgCl2, do I need? I'm very rusty on undergrad chemistry! Thank you!!!

12 December 2022 4,976 0 View

What is the best tree building software for inferring gene trees, specifically?

I have an alignment of about 40 proteins from marine invertebrates. I'd like to build a tree based on this alignment. I've used RAxML, but was wondering if there were other tree building software...

21 July 2021 8,458 2 View