Surya Paudel

3 Questions 4 Answers 0 Followers

Questions related from Surya Paudel

How to induce pBAD with arabinose in wild type E. coli?

I am trying to induce pBAD promotor. The plasmid with this promotor was inserted into a wild type E. coli by electrotransformation. I tried several different concentration of arabinose but no...

11 November 2019 3,287 3 View

Does a single copy gene of gfp have sufficient expression?

Does anyone have experience if a single copy gfp gene inserted into chromosome of bacteria is able to produce enough fluorescence to be visible in fluorescent microscope?

06 June 2016 7,599 2 View

Does the reverse complement of an insert affect on protein translation ?

I inserted a gene fragment into the chromosome which is supposed to give fluorescence under UV light. I checked the insertion with PCR which is positive. Sequencing showed that the fragment...

03 March 2016 921 3 View