Suresh Chandra Phulara

2 Questions 1 Answers 0 Followers

Questions related from Suresh Chandra Phulara

How to increase protein yield from transformed B. subtilis cells?

I have cloned B. subtilis endogenous genes in pHTO1 plasmid to overexpress them in B. subtilis. Real-time study is showing overexpression of the genes in B. subtilis, but there is no band...

09 September 2016 2,210 3 View

Does anyone have experience in recombinant plasmid isolation from transformed B. subtilis?

I had transformed B. subtilis and E. coli cells with same plasmid and transformed cells were allowed to grow on selection medium. Cells grown on selective medium were then subjected to plasmid...

11 November 2015 6,090 3 View