How to solubilize and purify eukaryotic membrane protein (ectodomain) in bacteria?

Hi, I am trying to express an eukaryotic membrane protein (only ectodomain, 60kDa, pI 5.6) in BL21DE3. I have played around with IPTG con. and temperature of induction to get the protein in...

19 December 2017 5,199 4 View